Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase.
Identifieur interne : 000B43 ( Main/Exploration ); précédent : 000B42; suivant : 000B44Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase.
Auteurs : S M Moukha [France] ; T J Dumonceaux ; E. Record ; F S ArchibaldSource :
- Gene [ 0378-1119 ] ; 1999.
Descripteurs français
- KwdFr :
- ADN recombiné (MeSH), Carbohydrate dehydrogenases (génétique), Champignons (enzymologie), Champignons (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Réaction de polymérisation en chaîne (MeSH), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Carbohydrate Dehydrogenases.
- enzymology : Fungi.
- genetics : Fungi.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Recombinant, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid.
Abstract
We have cloned and sequenced a gene encoding cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus (Pci). PCR primers that may recognize a homologous cdh were designed using regions of complete conservation of amino acid sequence within the known sequences of Trametes versicolor (Tv) and Phanerochaete chrysosporium (Pc) CDH. Upstream primers hybridized to regions encoding the heme domain, whereas downstream primers recognized highly conserved regions within the flavin domain. Eight different primer pairs yielded three PCR products close in size to the control amplification, which used cloned Tv cdh as template. The PCR products that were close to the control size were cloned, and one of these, a 1.8-kb product, was completely sequenced. The PCR product was highly homologous to both Tv and Pch cdh, and contained eight putative introns. The cloned product was used to isolate a full-length clone encoding CDH from a Pci genomic library. Pci cdh encoded a protein with 83% identity with Tv CDH and 74% identity with Pch CDH. Northern blot analysis revealed that Pci cdh was transcribed as a single mRNA species and was expressed in the presence of cellulose as the carbon source.
DOI: 10.1016/s0378-1119(99)00189-4
PubMed: 10393235
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Record</name></author><author><name sortKey="Archibald, F S" sort="Archibald, F S" uniqKey="Archibald F" first="F S" last="Archibald">F S Archibald</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10393235</idno><idno type="pmid">10393235</idno><idno type="doi">10.1016/s0378-1119(99)00189-4</idno><idno type="wicri:Area/Main/Corpus">000B38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B38</idno><idno type="wicri:Area/Main/Curation">000B38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B38</idno><idno type="wicri:Area/Main/Exploration">000B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase.</title><author><name sortKey="Moukha, S M" sort="Moukha, S M" uniqKey="Moukha S" first="S M" last="Moukha">S M Moukha</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique 163, Avenue de Luminy CP 925 13288, Marseille Cedex 09, France. serge.moukha@esil.univ-mrs.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique 163, Avenue de Luminy CP 925 13288, Marseille Cedex 09</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Dumonceaux, T J" sort="Dumonceaux, T J" uniqKey="Dumonceaux T" first="T J" last="Dumonceaux">T J Dumonceaux</name></author><author><name sortKey="Record, E" sort="Record, E" uniqKey="Record E" first="E" last="Record">E. Record</name></author><author><name sortKey="Archibald, F S" sort="Archibald, F S" uniqKey="Archibald F" first="F S" last="Archibald">F S Archibald</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Carbohydrate Dehydrogenases (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Recombinant (MeSH)</term><term>Fungi (enzymology)</term><term>Fungi (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (MeSH)</term><term>Carbohydrate dehydrogenases (génétique)</term><term>Champignons (enzymologie)</term><term>Champignons (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carbohydrate Dehydrogenases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carbohydrate dehydrogenases</term><term>Champignons</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>DNA, Recombinant</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN recombiné</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Réaction de polymérisation en chaîne</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have cloned and sequenced a gene encoding cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus (Pci). PCR primers that may recognize a homologous cdh were designed using regions of complete conservation of amino acid sequence within the known sequences of Trametes versicolor (Tv) and Phanerochaete chrysosporium (Pc) CDH. Upstream primers hybridized to regions encoding the heme domain, whereas downstream primers recognized highly conserved regions within the flavin domain. Eight different primer pairs yielded three PCR products close in size to the control amplification, which used cloned Tv cdh as template. The PCR products that were close to the control size were cloned, and one of these, a 1.8-kb product, was completely sequenced. The PCR product was highly homologous to both Tv and Pch cdh, and contained eight putative introns. The cloned product was used to isolate a full-length clone encoding CDH from a Pci genomic library. Pci cdh encoded a protein with 83% identity with Tv CDH and 74% identity with Pch CDH. Northern blot analysis revealed that Pci cdh was transcribed as a single mRNA species and was expressed in the presence of cellulose as the carbon source.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10393235</PMID><DateCompleted><Year>1999</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>234</Volume><Issue>1</Issue><PubDate><Year>1999</Year><Month>Jun</Month><Day>24</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase.</ArticleTitle><Pagination><MedlinePgn>23-33</MedlinePgn></Pagination><Abstract><AbstractText>We have cloned and sequenced a gene encoding cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus (Pci). PCR primers that may recognize a homologous cdh were designed using regions of complete conservation of amino acid sequence within the known sequences of Trametes versicolor (Tv) and Phanerochaete chrysosporium (Pc) CDH. Upstream primers hybridized to regions encoding the heme domain, whereas downstream primers recognized highly conserved regions within the flavin domain. Eight different primer pairs yielded three PCR products close in size to the control amplification, which used cloned Tv cdh as template. The PCR products that were close to the control size were cloned, and one of these, a 1.8-kb product, was completely sequenced. The PCR product was highly homologous to both Tv and Pch cdh, and contained eight putative introns. The cloned product was used to isolate a full-length clone encoding CDH from a Pci genomic library. Pci cdh encoded a protein with 83% identity with Tv CDH and 74% identity with Pch CDH. Northern blot analysis revealed that Pci cdh was transcribed as a single mRNA species and was expressed in the presence of cellulose as the carbon source.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Moukha</LastName><ForeName>S M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique 163, Avenue de Luminy CP 925 13288, Marseille Cedex 09, France. serge.moukha@esil.univ-mrs.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dumonceaux</LastName><ForeName>T J</ForeName><Initials>TJ</Initials></Author><Author ValidYN="Y"><LastName>Record</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Archibald</LastName><ForeName>F S</ForeName><Initials>FS</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AF081574</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004274">DNA, Recombinant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber><NameOfSubstance UI="D002237">Carbohydrate Dehydrogenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.99.18</RegistryNumber><NameOfSubstance UI="C019859">cellobiose-quinone oxidoreductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002237" MajorTopicYN="N">Carbohydrate Dehydrogenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004274" MajorTopicYN="N">DNA, Recombinant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1999</Year><Month>7</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1999</Year><Month>7</Month><Day>7</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1999</Year><Month>7</Month><Day>7</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10393235</ArticleId><ArticleId IdType="pii">S0378-1119(99)00189-4</ArticleId><ArticleId IdType="doi">10.1016/s0378-1119(99)00189-4</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><noCountry><name sortKey="Archibald, F S" sort="Archibald, F S" uniqKey="Archibald F" first="F S" last="Archibald">F S Archibald</name><name sortKey="Dumonceaux, T J" sort="Dumonceaux, T J" uniqKey="Dumonceaux T" first="T J" last="Dumonceaux">T J Dumonceaux</name><name sortKey="Record, E" sort="Record, E" uniqKey="Record E" first="E" last="Record">E. Record</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Moukha, S M" sort="Moukha, S M" uniqKey="Moukha S" first="S M" last="Moukha">S M Moukha</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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